Abstract
We investigated the expression of muscarinic acetylcholine receptors (mAChRs) and their possible involvement in the regulation of cell proliferation in four colon cancer cell lines (SNU-61, SNU-81, SNU-407, and SNU-1033) derived from Korean colon carcinoma patients. A ligand binding assay showed that all four cell lines expressed mAChRs. Treatment of the four cell lines with the cholinergic agonist carbachol led to the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2). In SNU-407 cells, carbachol significantly stimulated cell proliferation, which could be abolished by the muscarinic antagonist atropine and the ERK1/2 kinase inhibitor PD98059. These results indicate that mAChRs specifically mediate the proliferation of SNU-407 colon cancer cells via the ERK1/2 pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Atropine / pharmacology
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Carbachol / pharmacology
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Carcinoma / metabolism
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Carcinoma / pathology*
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Cell Line, Tumor*
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Cell Proliferation* / drug effects
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Colonic Neoplasms / metabolism
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Colonic Neoplasms / pathology*
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Flavonoids / pharmacology
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Humans
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Mitogen-Activated Protein Kinase 1 / antagonists & inhibitors
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Mitogen-Activated Protein Kinase 1 / physiology
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Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors
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Mitogen-Activated Protein Kinase 3 / physiology
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Muscarinic Agonists / pharmacology
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Muscarinic Antagonists / pharmacology
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Receptors, Muscarinic / metabolism
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Receptors, Muscarinic / physiology*
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Up-Regulation
Substances
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Flavonoids
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Muscarinic Agonists
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Muscarinic Antagonists
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Receptors, Muscarinic
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Atropine
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Carbachol
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one