Abstract
Phosphoglycerate kinase 2 (PGK2) is a germ cell-specific protein whose mRNA is translationally regulated in the mammalian testis. Using RNA affinity chromatography with the 3'-untranslated region (UTR) of Pgk2 mRNA and adult testis extracts, several associated proteins including a novel isoform of the AU-rich element RNA-binding protein and KH-type splicing regulatory protein (KSRP) were identified. KSRP, a protein of approximately 75 kDa, is widely expressed in somatic and germ cells where it is primarily nuclear. In addition to the approximately 75-kDa KSRP, a approximately 52-kD KSRP, t-KSRP, is present in the cytoplasm of a subpopulation of germ cells. t-KSRP binds directly to a 93-nt sequence (designated the F1 region) of the 3'-UTR of the Pgk2 mRNA and destabilizes Pgk2 mRNA constructs in testis extracts and in transfected cells. We conclude that this testicular variant of the multifunctional nucleic acid-binding protein, KSRP, serves as a decay-promoting factor for Pgk2 mRNA in male germ cells.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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3' Untranslated Regions / chemistry
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3' Untranslated Regions / metabolism
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Animals
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Binding Sites
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Cytoplasm / metabolism
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Genetic Variation
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HeLa Cells
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Humans
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Isoenzymes / genetics*
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Isoenzymes / metabolism
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Male
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Meiosis
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Mice
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Nerve Tissue Proteins / metabolism
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Phosphoglycerate Kinase / genetics*
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Phosphoglycerate Kinase / metabolism
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Polypyrimidine Tract-Binding Protein / metabolism
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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RNA Stability*
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RNA, Messenger / metabolism*
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RNA-Binding Proteins / isolation & purification
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RNA-Binding Proteins / metabolism*
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Testis / enzymology
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Testis / metabolism*
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Trans-Activators / isolation & purification
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Trans-Activators / metabolism*
Substances
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3' Untranslated Regions
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Isoenzymes
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Khsrp protein, mouse
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Nerve Tissue Proteins
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Protein Isoforms
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Ptbp2 protein, mouse
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RNA, Messenger
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RNA-Binding Proteins
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Trans-Activators
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Polypyrimidine Tract-Binding Protein
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Phosphoglycerate Kinase
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phosphoglycerate kinase, testis specific