Abstract
Selected PvDBP-derived synthetic peptides were tested in competition assays with HLA molecules in order to identify and evaluate their binding to a wide range of MHC class II molecules. Binding was evaluated as the peptide's ability to displace the biotinylated control peptide (HA(306-318)) and was detected by a conventional ELISA. Thus, one epitope for the HLA-DR1 molecule, two epitopes for the HLA-DR4 molecule, six epitopes for the HLA-DR7 molecule and three epitopes for the HLA-DR11 molecule displaying a high binding percentage (above 50%) were experimentally obtained. The in vitro results were compared with the epitope prediction results. Two peptides behaved as universal epitopes since they bound to a larger number of HLA-DR molecules. Given that these peptides are located in the conserved PvDBP region II, they could be considered good candidates to be included in the design of a synthetic vaccine against Plasmodium vivax malaria.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Protozoan / chemistry
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Antigens, Protozoan / genetics
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Antigens, Protozoan / immunology*
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Epitope Mapping
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HLA-DR Antigens / immunology
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HLA-DRB1 Chains
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Immunodominant Epitopes / chemistry
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Immunodominant Epitopes / genetics
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Immunodominant Epitopes / immunology*
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Malaria Vaccines / chemistry
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Malaria Vaccines / genetics
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Malaria Vaccines / immunology
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Molecular Sequence Data
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Peptides / genetics
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Peptides / immunology
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Plasmodium vivax / immunology*
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Protein Conformation
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Protozoan Proteins / chemistry
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Protozoan Proteins / genetics
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Protozoan Proteins / immunology*
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Receptors, Cell Surface / chemistry
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / immunology*
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Sequence Alignment
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Vaccines, Synthetic / chemistry
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology
Substances
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Antigens, Protozoan
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Duffy antigen binding protein, Plasmodium
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HLA-DR Antigens
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HLA-DRB1 Chains
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Immunodominant Epitopes
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Malaria Vaccines
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Peptides
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Protozoan Proteins
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Receptors, Cell Surface
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Vaccines, Synthetic