Objective: To study the optimum extraction process of total flavonoids in Herba Desmodii Styracifolii.
Methods: Using the extraction yield of total flavonoids as the assessment index, some important factors in the experimental orthogonal design, such as volume of extraction water, extracting time and times, alcohol concentration were studied. The total flavonoids content extracted from Herba Desmodii Styracifolii was determined by Spectrophotometric method at 272 nm wavelength.
Results: The optimum ethanol extracting process conditions were as follows: adding 15 fold of water extracting three times, heating for 1.5 hours, the use of alcohol concentration of 50%. Rutin standard sample showed a good linear relationship at the range of 4.36 - 21.8 microg/ml and the average recovery was 98.92%, and RSD was 1.85%.
Conclusion: The optimum extraction process of total flavonoids in Herba Desmodii Styracifolii is reliable and this method is easy and accurate so that it can be used for the extraction of total flavonoids in Herba Desmodii Styracifolii.