Abstract
Solid-state two-dimensional refocused INADEQUATE MAS NMR experiments resolve distinct helical and beta-sheet conformational environments for both alanine and glycine in Nephila clavipes dragline silk fibers; the fraction of alanine and glycine in beta-sheet structures is determined to be 82% +/- 4% and 28% +/- 5%, respectively.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Alanine / chemistry*
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Animals
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Carbon Isotopes
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Glycine / chemistry*
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Magnetic Resonance Spectroscopy / methods*
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Magnetic Resonance Spectroscopy / standards
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Protein Structure, Secondary
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Reference Standards
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Silk / chemistry*
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Spiders*
Substances
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Carbon Isotopes
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Silk
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Alanine
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Glycine