A novel L-amino acid ligase is encoded by a gene in the phaseolotoxin biosynthetic gene cluster from Pseudomonas syringae pv. phaseolicola 1448A

Toshinobu Arai; Kuniki Kino

doi:10.1271/bbb.80439

A novel L-amino acid ligase is encoded by a gene in the phaseolotoxin biosynthetic gene cluster from Pseudomonas syringae pv. phaseolicola 1448A

Biosci Biotechnol Biochem. 2008 Nov;72(11):3048-50. doi: 10.1271/bbb.80439. Epub 2008 Nov 7.

Authors

Toshinobu Arai¹, Kuniki Kino

Affiliation

¹ Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, Tokyo, Japan.

PMID: 18997422
DOI: 10.1271/bbb.80439

Abstract

In the phaseolotoxin biosynthetic gene cluster of Pseudomonas syringae pv. phaseolicola 1448A, the PSPPH_4299 gene encodes a novel L-amino acid ligase. The PSPPH_4299 protein synthesized various hetero-dipeptides containing basic amino acids in an ATP-dependent manner, and also synthesized alanyl-homoarginine, part of the phaseolotoxin scaffold.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dipeptides / biosynthesis
Genes, Bacterial / genetics*
Multigene Family / genetics*
Ornithine / analogs & derivatives*
Ornithine / biosynthesis
Peptide Synthases / genetics*
Peptide Synthases / metabolism
Pseudomonas syringae / enzymology*
Pseudomonas syringae / genetics*
Substrate Specificity

Substances

Dipeptides
phaseolotoxin
Ornithine
Peptide Synthases