Improvement of L-lactate production by CYB2 gene disruption in a recombinant Saccharomyces cerevisiae strain under low pH condition

Aki Ookubo; Takashi Hirasawa; Katsunori Yoshikawa; Keisuke Nagahisa; Chikara Furusawa; Hiroshi Shimizu

doi:10.1271/bbb.80493

Improvement of L-lactate production by CYB2 gene disruption in a recombinant Saccharomyces cerevisiae strain under low pH condition

Biosci Biotechnol Biochem. 2008 Nov;72(11):3063-6. doi: 10.1271/bbb.80493. Epub 2008 Nov 7.

Authors

Aki Ookubo¹, Takashi Hirasawa, Katsunori Yoshikawa, Keisuke Nagahisa, Chikara Furusawa, Hiroshi Shimizu

Affiliation

¹ Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Japan.

PMID: 18997405
DOI: 10.1271/bbb.80493

Abstract

Using a DNA microarray, we found that expression of the genes related to lactate metabolism was upregulated in a lactate-producing recombinant Saccharomyces cerevisiae strain. Disruption of the CYB2 gene encoding L-lactate dehydrogenase improved the L-lactate production by S. cerevisiae under low pH condition.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Genetic Engineering*
Humans
Hydrogen-Ion Concentration
L-Lactate Dehydrogenase / genetics*
L-Lactate Dehydrogenase / metabolism
Lactic Acid / biosynthesis*
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / metabolism*

Substances

Lactic Acid
L-Lactate Dehydrogenase