Abstract
Herein we describe the cloning, functional expression and initial characterization of ORF36 from Micromonospora carbonacae var. africana and rubN8 from Streptomyces achromogenes var. rubradiris. The purified enzymes play the same role, the double-oxidation of TDP-evernosamine to TDP-evernitrosose in the everninomycin and rubradirin pathways, respectively.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Oxidoreductases / metabolism*
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Bacterial Proteins / metabolism*
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Carbohydrates / biosynthesis*
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Carbohydrates / chemistry*
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Chromatography, High Pressure Liquid
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Mass Spectrometry
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Micromonospora / enzymology
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Molecular Structure
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Nitroso Compounds / chemistry*
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Nitroso Compounds / metabolism*
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Oxidation-Reduction
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Streptomyces / enzymology
Substances
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Bacterial Proteins
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Carbohydrates
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Nitroso Compounds
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Amino Acid Oxidoreductases