A brief summary of the historical developments relating to plant distribution and aquatic macrophyte-nutrient indices provided a means of assessing the general context and validity of previous assumptions. This has particular current relevance because of the prominent use of bioindicators for defining nutrient enrichment. A survey of 161 sites distributed across two broadly contrasting groups of rivers (circum-neutral versus alkaline) recorded 110 species of aquatic macrophytes and these have been statistically analyzed to (i) rank and separate the individual effects of local environmental conditions and spatial isolation on species distribution in the two contrasting groups of sites; (ii) calculate a macrophyte index based on plant cover and species indicator values (Mean Trophic Rank, MTR); and finally (iii) investigate the implications for biomonitoring. Chemical, physical and hydrological site attributes together with spatial isolation, each explained a significant and at least partially independent influence over plant species distribution. It was extremely difficult, however, to separate the single effects of different site attributes on plant distribution. While some plant species are more restricted to certain environmental conditions, many appeared indifferent to the range of those being tested. The role played by nutrients (nitrogen (N) and phosphorus (P)) were either mostly indistinguishable from other site attributes (e.g., nitrate from conductivity) or subordinate (e.g., soluble reactive phosphorus, ammonium). It is therefore very unlikely that macrophyte species composition could provide a reliable bioindicator of the surrounding nutrient (N, P) status. The calculation of the plant index illustrated this unreliability by showing that strong correlations existed with many environmental variables, not just inorganic N and P.