Specific interaction of TFII-I with an upstream element on the HIV-1 LTR regulates induction of latent provirus

FEBS Lett. 2008 Nov 26;582(28):3903-8. doi: 10.1016/j.febslet.2008.10.032. Epub 2008 Oct 29.

Abstract

RBF-2 is a factor comprised of a USF1/2 heterodimer, whose association with a highly conserved upstream element (RBEIII) on the HIV-1 LTR requires a co-factor TFII-I. We have identified specific nucleotides, immediately 3' of RBEIII that are required for stable association of TFII-I with this region of the LTR. Mutations that inhibit interaction of TFII-I with DNA also prevent stimulation of USF binding to RBEIII, and render the integrated LTR unresponsive to T cell signaling. These results demonstrate an essential role of TFII-I bound at an upstream LTR element for viral replication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Gene Expression Regulation, Viral*
  • HIV Long Terminal Repeat* / genetics
  • HIV-1 / genetics
  • HIV-1 / physiology*
  • Humans
  • Jurkat Cells
  • Mutation
  • Proviruses / genetics
  • Proviruses / physiology*
  • Transcription Factors, TFII / metabolism*
  • Upstream Stimulatory Factors / metabolism
  • Virus Activation / genetics*

Substances

  • GTF2I protein, human
  • Transcription Factors, TFII
  • Upstream Stimulatory Factors