Abstract
A new water-soluble oligo(phenylenevinylene) containing a tyrosine unit (OPV-Tyr) was synthesized as a fluorescent probe to optically detect tyrosinase activity. Upon the addition of tyrosinase, the tyrosine moiety was oxidized to quinone, which quenched the fluorescence of OPV-Tyr via intramolecular electron transfer from the phenylenevinylene unit to the quinone site. OPV-Tyr was elaborated to detect tyrosinase activity both in aqueous buffer solution and in agarose gel.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Enzyme Assays / methods*
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry
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Monophenol Monooxygenase / metabolism*
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Polyethylene Glycols / chemistry
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Polyvinyls / chemical synthesis*
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Polyvinyls / chemistry*
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Solubility
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Spectrometry, Fluorescence
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Tyrosine / chemistry*
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Water / chemistry*
Substances
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Fluorescent Dyes
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Polyvinyls
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oligo(p-phenylene vinylene)
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Water
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Polyethylene Glycols
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Tyrosine
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Monophenol Monooxygenase