[The study on growth and proliferation of neural stem cells from rats in vitro]

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2008 Aug;22(16):747-50.
[Article in Chinese]

Abstract

Objective: To investigate culturing neural stem cells (NSCs) from rat embryos in vitro and to observe their growth and differentiation.

Method: NSCs were isolated from hippocampus of SD rat embryos (P16-P18) and cultured in DMEM/F12 medium containing EGF, bFGF, B27. To observe process of cell proliferation by microscope and identify cell types by immunocytochemical analyses after differentiation.

Result: NSCs grew well in serum-free conditional medium and their cell bodies present transparent with good refraction at about eighth day. After differentiation, the cells demonstrated NSE and GFAP immunoreactive.

Conclusion: NSCs were cultured well in serum-free conditional medium and they could be induced to differentiate into neurons and astrocytes in serum conditional medium.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation*
  • Cell Proliferation
  • Cells, Cultured
  • Culture Media, Serum-Free
  • Embryo, Mammalian
  • Female
  • Hippocampus / cytology
  • Hippocampus / embryology
  • Multipotent Stem Cells / cytology
  • Neural Stem Cells / cytology*
  • Pregnancy
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Culture Media, Serum-Free