The development of sequential injection analysis coupled with lab-on-valve for copper determination

Tapparath Leelasattarathkul; Saisunee Liawruangrath; Mongkon Rayanakorn; Winai Oungpipat; Boonsom Liawruangrath

doi:10.1016/j.talanta.2006.03.047

The development of sequential injection analysis coupled with lab-on-valve for copper determination

Talanta. 2006 Oct 15;70(3):656-60. doi: 10.1016/j.talanta.2006.03.047. Epub 2006 May 2.

Authors

Tapparath Leelasattarathkul¹, Saisunee Liawruangrath, Mongkon Rayanakorn, Winai Oungpipat, Boonsom Liawruangrath

Affiliation

¹ Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Division of Analytical Chemistry, Department of Chemical Technology, Rajamanjala University of Technology Krungthep, Bangkok 10120, Thailand.

PMID: 18970823
DOI: 10.1016/j.talanta.2006.03.047

Abstract

A sequential injection analysis (SIA) using lab-on-valve with air segmentation and spectrophotometric detection was designed for copper(II) determination. It is based on the reaction of copper(II) and 2-carboxy-2'-hydroxy-5'-sulfoformazyl benzene (Zincon) in a weak alkaline solution between the air zones. Beer's Law was obeyed over the range of 0.1-2.0mgL(-1) copper(II) with a correlation coefficient 0.9985 and a slope of 0.2893 absorbance unit/mgL(-1). The relative standard deviation was 2.0% for a series of 10 measurements of 0.5mgL(-1) copper(II) solution. The detection limit (3 S/N) and the limit of quantification (LOQ) were 0.05 and 0.17mgL(-1) respectively. This method has been successfully applied to determination of copper(II) in wastewater with a sample throughput of 120h(-1). The method is superior to the batchwise method in that it provides fully automation, rapidity, less reagents and sample consumption with little waste generation.