Electrochemiluminescent behaviors of alkaloids and tris(2,2'-bipyridine) ruthenium in organically modified silicate film

Li Zhao; Ying Tao; Xiaoqing Yang; Liyan Zhang; Munetaka Oyama; Xi Chen

doi:10.1016/j.talanta.2005.11.043

Electrochemiluminescent behaviors of alkaloids and tris(2,2'-bipyridine) ruthenium in organically modified silicate film

Talanta. 2006 Aug 15;70(1):104-10. doi: 10.1016/j.talanta.2005.11.043. Epub 2006 Jan 18.

Authors

Li Zhao¹, Ying Tao, Xiaoqing Yang, Liyan Zhang, Munetaka Oyama, Xi Chen

Affiliation

¹ Department of Chemistry and Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

PMID: 18970736
DOI: 10.1016/j.talanta.2005.11.043

Abstract

Electrogenerated chemiluminescences (ECLs) of alkaloids, such as berberine, trigonelline, allantoin and betaine, were studied in an aqueous alkaline buffer solution (pH 9.5), based on tris(2,2'-bipyridine)ruthenium(II) [Ru(bpy)(3)(2+)] immobilized in organically modified silicates (ORMOSILs) film on a glassy carbon electrode (GCE). The immobilized Ru(bpy)(3)(2+) showed good electrochemical and photochemical activities. In a flow system, the eluted alkaloids were oxidized on the modified GCE, and reacted with immobilized Ru(bpy)(3)(2+) at the potential of +1.50V (versus Ag/AgCl). The luminescence with lambda(max) 610nm was caused by a reaction of electrolytically formed Ru(bpy)(3)(3+) with an oxidized amine group to generate Ru(bpy)(3)(2+*). The determination limit was 5x10(-6)molL(-1), 8x10(-6)molL(-1), 2.0x10(-5)molL(-1) and 5.0x10(-5)molL(-1) for berberine, trigonelline, allantoin and betaine at S/N 3, respectively. In addition, the factors affecting the determination of the four alkaloids were also studied.