Fluorescence resonance energy transfer in doubly-quantum dot labeled IgG system

Qiang Ma; Xing-Guang Su; Xin-Yan Wang; Yi Wan; Chun-Lei Wang; Bai Yang; Qin-Han Jin

doi:10.1016/j.talanta.2005.04.036

Fluorescence resonance energy transfer in doubly-quantum dot labeled IgG system

Talanta. 2005 Oct 31;67(5):1029-34. doi: 10.1016/j.talanta.2005.04.036. Epub 2005 May 31.

Authors

Qiang Ma¹, Xing-Guang Su, Xin-Yan Wang, Yi Wan, Chun-Lei Wang, Bai Yang, Qin-Han Jin

Affiliation

¹ Institute for Miniature Analytical Instrumentation, College of Chemistry, Jilin University, Changchun 130023, PR China.

PMID: 18970275
DOI: 10.1016/j.talanta.2005.04.036

Abstract

The mouse immunoglobulin G (mouse IgG) as a kind of bio-molecule was labeled with two different luminescent colloidal semiconductor quantum dots (QDs), green-emitting CdTe quantum dots and red-emitting CdTe quantum dots in this work. As a result of the fluorescence resonance energy transfer (FRET) between the two different sizes nanoparticles with mouse IgG as the binding bridge, a significant enhancement of the emission of the red-emitting CdTe quantum dots and the corresponding quenching of the emission of green-emitting CdTe quantum dots were observed. The relationship between the concentration of the mouse immunoglobulin G and the fluorescence intensity ratio (I(a)/I(d)) of acceptors and donors was studied also. Under optimal conditions, the calibration graph is linear over the range of 0.1-20.0mg/L mouse IgG.