Electrooxidative polymerization of phenothiazine derivatives on screen-printed carbon electrode and its application to determine NADH in flow injection analysis system

Qiang Gao; Weidong Wang; Ying Ma; Xiurong Yang

doi:10.1016/j.talanta.2003.08.017

Electrooxidative polymerization of phenothiazine derivatives on screen-printed carbon electrode and its application to determine NADH in flow injection analysis system

Talanta. 2004 Feb 27;62(3):477-82. doi: 10.1016/j.talanta.2003.08.017.

Authors

Qiang Gao¹, Weidong Wang, Ying Ma, Xiurong Yang

Affiliation

¹ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Renmin Street 159, Changchun, Jilin 130022, China.

PMID: 18969321
DOI: 10.1016/j.talanta.2003.08.017

Abstract

The electrooxidation polymerization of phenothiazine derivatives, including azure A and toluidine blue O, has been studied at screen-printed carbon electrodes in neutral phosphate buffer. Both compounds yield strongly adsorbed electroactive polymer with reversible behavior and formal potentials closed to 0.04V at pH 6.9. The modified electrodes exhibited good stability and electrocatalysis for NADH oxidation in phosphate buffer (pH 6.9), with an overpotential of more than 500mV lower than that of the bare electrodes. Further, the modified screen-printed carbon electrodes were found to be promising as an amperometric detector for the flow injection analysis (FIA) of NADH, typically with a dynamic range of 0.5-100muM.