HPLC Determination of some antibilharzial antimonials by extraction of antimony

N A El-Rabbat; H H Farag; M E El-Kommos; I H Refaat

doi:10.1016/0039-9140(90)80259-i

HPLC Determination of some antibilharzial antimonials by extraction of antimony

Talanta. 1990 Sep;37(9):951-4. doi: 10.1016/0039-9140(90)80259-i.

Authors

N A El-Rabbat¹, H H Farag, M E El-Kommos, I H Refaat

Affiliation

¹ Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Assiut, Assiut, Egypt.

PMID: 18965048
DOI: 10.1016/0039-9140(90)80259-i

Abstract

The antimonial drug (antimony potassium tartrate, antimony piperazine tartrate or antimony lithium thiomaleate) in aqueous solution or biological fluid is treated with sodium diethyldithiocarbamate in the presence of a suitable masking reagent, the pH is adjusted to 9 +/- 0.5. and the antimony complex extracted with n-hexane and determined by reversed-phase HPLC with an ODS column and detection at 254 nm. The limits of detection are 20 ng (for antimony potassium tartrate and antimony lithium thiomaleate) and 16 ng (for antimony piperazine tartrate).