Assembly of combinatorial antibody libraries on phage surfaces: the gene III site

C F Barbas 3rd; A S Kang; R A Lerner; S J Benkovic

doi:10.1073/pnas.88.18.7978

Assembly of combinatorial antibody libraries on phage surfaces: the gene III site

Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7978-82. doi: 10.1073/pnas.88.18.7978.

Authors

C F Barbas 3rd¹, A S Kang, R A Lerner, S J Benkovic

Affiliation

¹ Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.

Abstract

A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carboxyl-terminal domain of the gene III protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Antibodies, Bacterial / genetics
Antibody Diversity
Base Sequence
Binding Sites, Antibody
Capsid / genetics*
Cloning, Molecular
Gene Expression
Gene Library*
Genetic Vectors
Immunoglobulin Fab Fragments / genetics*
Molecular Sequence Data
Oligonucleotides / chemistry
Recombinant Fusion Proteins
Tetanus Toxoid / immunology

Substances

Antibodies, Bacterial
Immunoglobulin Fab Fragments
Oligonucleotides
Recombinant Fusion Proteins
Tetanus Toxoid