Apoptosis in detached corn root cap cells under fluorescent microscope was studied. Homokaryon maize B37 inbred lines was tested and the detached corn root cap cells with cell wall might be stained using Hoechst33342. Apoptosis was found under fluorescence microscope treated by culture filtrate of HMC toxin. The sensitivity of HMC toxin to C cytoplasm was greater than N cytoplasm and there was more resistance response in detached corn root cap cells of N cytoplasm than C cytoplasm. Besides, apoptosis did not coincide at different processing times. These indicated that special disease response occurred in the course of apoptosis in detached corn root cap cells of C cytoplasm maize led by HMC which may be collective result of nucleus and cytoplasm.