Masculinization of XX Drosophila transgenic flies expressing the Ceratitis capitata DoublesexM isoform

Int J Dev Biol. 2008;52(8):1051-7. doi: 10.1387/ijdb.082657gs.

Abstract

The Doublesex (DSX) transcription factor regulates somatic sexual differentiation in Drosophila melanogaster. Female and male isoforms (DSXF and DSXM) are produced due to sex-specific RNA splicing. Here we show that in the distantly related dipteran Ceratitis capitata, the DSXM male-specific isoform is conserved and able to induce masculinization of both somatic and germline tissues when ectopically expressed in XX Drosophila transgenic individuals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Binding Sites / genetics
  • Ceratitis capitata / genetics*
  • Cloning, Molecular
  • DNA Primers / genetics
  • DNA-Binding Proteins / genetics*
  • Drosophila Proteins / genetics*
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / growth & development*
  • Female
  • Genes, Insect
  • Insect Proteins / genetics*
  • Male
  • Molecular Sequence Data
  • Phenotype
  • Protein Isoforms / genetics
  • Sequence Homology, Amino Acid
  • Sex Differentiation / genetics
  • Species Specificity

Substances

  • 3' Untranslated Regions
  • DNA Primers
  • DNA-Binding Proteins
  • DSX protein, Drosophila
  • Drosophila Proteins
  • Insect Proteins
  • Protein Isoforms