This in vivo study investigated interexaminer reliability in the clinical measurement of L*C*h* values using a spectrophotometer (Shadepilot, DeguDent), which provides laminar measurement. Thirty incisors were measured by 3 trained clinicians. Intraclass correlation coefficient was used to assess reliability. Additionally, the range of differences between the measurements by all examiners for each subject was calculated to assess the clinical impact of the differences. Agreement was acceptable to excellent for all measurements. Additionally, the reliability of the measurement of L* and C* was excellent (all measurements < 5). Laminar spectrophotometric measurement seems to be superior to punctiform measurement.