Abstract
A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks.
Publication types
-
Comparative Study
-
Evaluation Study
MeSH terms
-
Humans
-
Mumps / diagnosis*
-
Mumps / virology
-
Mumps virus / genetics
-
Mumps virus / isolation & purification*
-
RNA, Viral / genetics
-
RNA, Viral / isolation & purification*
-
Reverse Transcriptase Polymerase Chain Reaction / methods*
-
Ribonuclease P / genetics
-
Sensitivity and Specificity
-
Viral Fusion Proteins / genetics
Substances
-
RNA, Viral
-
Viral Fusion Proteins
-
Ribonuclease P