ABSTRACT We investigated the relationship between physical characteristics of artificial surfaces, spore attachment, and spore germination in Colletotrichum graminicola. Surface hydrophobicity and surface rigidity were both signals for breaking dormancy and initiating spore germination, but spore attachment alone was not an important inducing signal. The presence of a carbon source overrode the necessity for a rigid, hydrophobic substrate for spore germination. Spore attachment was typically stronger to more hydrophobic surfaces, but certain hydrophilic surfaces also proved to be good substrates for spore attachment. In contrast to spore germination, appressorial induction was more dependent on attachment to a rigid substrate than it was on surface hydrophobicity. Appressoria were induced efficiently on hydrophilic surfaces, as long as there was significant conidial attachment to those surfaces.