Population data on 10 non-CODIS STR loci in Japanese population using a newly developed multiplex PCR system

J Forensic Leg Med. 2008 Nov;15(8):519-23. doi: 10.1016/j.jflm.2008.04.001. Epub 2008 Jun 12.

Abstract

This paper describes a newly devised autosomal short tandem repeat (STR) multiplex polymerase chain reaction (PCR) systems for 10 loci (D1S1656, D2S1353, D8S1132, D12S1090, D14S608, D18S535, D19S253, D20S480, D21S226, and D22S689) unlinked to the core STR loci (non-CODIS loci). Of 252 samples taken from the Japanese population, PCR products ranged in length from 107 bp to 319 bp. No significant deviations from Hardy-Weinberg equilibrium were observed at any of the 10 loci. The accumulated power of discrimination and power of exclusion for the 10 loci were 0.999999999998 and 0.99991, respectively. We conclude that the present multiplex system for the 10 non-CODIS loci represents a powerful tool for forensic applications.

Publication types

  • Comparative Study

MeSH terms

  • Asian People / genetics*
  • Chromosome Mapping / methods*
  • DNA Fingerprinting
  • Family*
  • Female
  • Forensic Medicine*
  • Gene Frequency
  • Genetic Markers
  • Genetics, Population*
  • Genotype
  • Humans
  • Linkage Disequilibrium
  • Male
  • Microsatellite Repeats / genetics*
  • Molecular Sequence Data
  • Paternity
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / statistics & numerical data
  • Polymorphism, Genetic*
  • Sequence Analysis, DNA
  • Tandem Repeat Sequences
  • White People / genetics

Substances

  • Genetic Markers