Enzymatic methylation, which is catalyzed by the large number of O-methyltransferases (OMTs), is one of the important reactions in the flow of primary and (or) secondary metabolism. In a previous study, the gene TaOMT1 was induced by Hessian fly infestation of a wheat-rye translocation line. In this study we considered other wheat OMT genes - TaOMT3, TaOMT4, and TaOMT5 - using a bioinformatics approach and examined the TaOMT genes for their genomic organization, tissue-specific expression, responses to abiotic stresses and hormones, and cis-elements. There appeared to be a homoeologous relationship between TaOMT4 (6DS) and TaOMT5 (6BS), whereas TaOMT1 and TaOMT3 were placed on chromosome arms 7BL and 5DL, respectively. Differences in the tissue-specific, constitutive, and stress-inducible expression patterns among the TaOMT genes were found in both healthy and stressed plants. A number of cis-elements, which are potentially correlated with the responses of the TaOMT genes, were detected in the analysis of the TaOMT promoter sequences. In addition, evolutionary perspectives of the TaOMT genes are discussed. The nucleotide sequences have been deposited in the GenBank database under accession Nos. AAP23942 (TaCOMT1), EF423610 (TaOMT5), EF423611 (TaOMT4), EF423612 (TaOMT3), EU831287 (5' upstream of TaOMT1), EU831288 (5' upstream of TaOMT3), EU831289 (5' upstream of TaOMT4), and EU831290 (5' upstream of TaOMT5).