Increased detectability of plasma HIV-1 RNA after introduction of a new assay and altered specimen-processing procedures

Peter F Rebeiro; Asghar Kheshti; Sally S Bebawy; Samuel E Stinnette; Husamettin Erdem; Yi-Wei Tang; Timothy R Sterling; Stephen P Raffanti; Richard T D'Aquila

doi:10.1086/592693

Increased detectability of plasma HIV-1 RNA after introduction of a new assay and altered specimen-processing procedures

Clin Infect Dis. 2008 Nov 15;47(10):1354-7. doi: 10.1086/592693.

Authors

Peter F Rebeiro¹, Asghar Kheshti, Sally S Bebawy, Samuel E Stinnette, Husamettin Erdem, Yi-Wei Tang, Timothy R Sterling, Stephen P Raffanti, Richard T D'Aquila

Affiliation

¹ Division of Infectious Diseases, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2582, USA.

PMID: 18922071
PMCID: PMC2605467
DOI: 10.1086/592693

Abstract

After changes to assay and specimen-processing methods, plasma human immunodeficiency virus type 1 (HIV-1) RNA was frequently detectable in patients who previously had well-suppressed HIV-1 RNA levels. This artifact is attributable to shipping frozen plasma in primary plasma preparation tubes and is not caused by the HIV-1 RNA detection assay; it can be avoided by shipping plasma in a secondary tube.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Freezing
HIV Infections / diagnosis
HIV Infections / virology*
HIV-1 / genetics
HIV-1 / isolation & purification*
Humans
Plasma / virology
RNA, Viral / blood*
Specimen Handling / methods*
Viral Load / methods*

Substances

RNA, Viral

Abstract

Publication types

MeSH terms

Substances

Grants and funding