The specific activation of TRPC4 by Gi protein subtype

Biochem Biophys Res Commun. 2008 Dec 12;377(2):538-543. doi: 10.1016/j.bbrc.2008.10.012. Epub 2008 Oct 12.

Abstract

The classical type of transient receptor potential channel (TRPC) is a molecular candidate for Ca(2+)-permeable cation channels in mammalian cells. Especially, TRPC4 has the similar properties to Ca(2+)-permeable nonselective cation channels (NSCCs) activated by muscarinic stimulation in visceral smooth muscles. In visceral smooth muscles, NSCCs activated by muscarinic stimulation were blocked by anti-Galphai/o antibodies. However, there is still no report which Galpha proteins are involved in the activation process of TRPC4. Among Galpha proteins, only Galphai protein can activate TRPC4 channel. The activation effect of Galphai was specific for TRPC4 because Galphai has no activation effect on TRPC5, TRPC6 and TRPV6. Coexpression with muscarinic receptor M2 induced TRPC4 current activation by muscarinic stimulation with carbachol, which was inhibited by pertussis toxin. These results suggest that Galphai is involved specifically in the activation of TRPC4.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium Channels / genetics
  • Calcium Channels / metabolism
  • Cell Line
  • GTP-Binding Protein alpha Subunit, Gi2 / antagonists & inhibitors
  • GTP-Binding Protein alpha Subunit, Gi2 / genetics
  • GTP-Binding Protein alpha Subunit, Gi2 / metabolism*
  • Guanosine 5'-O-(3-Thiotriphosphate) / metabolism
  • Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology
  • Humans
  • Mice
  • Receptor, Muscarinic M2 / genetics
  • TRPC Cation Channels / agonists*
  • TRPC Cation Channels / metabolism
  • TRPV Cation Channels / genetics
  • TRPV Cation Channels / metabolism

Substances

  • Calcium Channels
  • Receptor, Muscarinic M2
  • TRPC Cation Channels
  • TRPC4 ion channel
  • TRPV Cation Channels
  • TRPV6 protein, human
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • GTP-Binding Protein alpha Subunit, Gi2