Enhancement of laser-induced fluorescence at 473 nm excitation with subwavelength resonant waveguide gratings

Opt Express. 2008 Oct 13;16(21):16364-70. doi: 10.1364/oe.16.016364.

Abstract

A dielectric subwavelength resonant waveguide grating was designed and fabricated in order to enhance fluorescence of biomolecules. More than 80 times higher laser-induced fluorescence yield was observed from enhanced green fluorescence protein on the structure when compared to same material on a flat surface.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Equipment Design
  • Equipment Failure Analysis
  • Image Enhancement / instrumentation*
  • Lasers*
  • Lighting / instrumentation*
  • Microscopy, Fluorescence / instrumentation*
  • Refractometry / instrumentation*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence / instrumentation*