PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria

Appl Environ Microbiol. 2008 Dec;74(23):7422-6. doi: 10.1128/AEM.01369-08. Epub 2008 Oct 10.

Abstract

We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the P(BAD) promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • AraC Transcription Factor / genetics
  • Burkholderia / genetics*
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Escherichia coli Proteins / genetics
  • Gene Expression
  • Genes, Bacterial*
  • Genetic Vectors*
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Pseudomonas / genetics*

Substances

  • AraC Transcription Factor
  • AraC protein, E coli
  • Escherichia coli Proteins

Associated data

  • GENBANK/EU603324
  • GENBANK/EU603325
  • GENBANK/EU603326
  • GENBANK/EU603327