Synthesis of 8-bromo-, 8-methyl- and 8-phenyl-dATP and their polymerase incorporation into DNA

Org Biomol Chem. 2008 Oct 21;6(20):3657-60. doi: 10.1039/b811935j. Epub 2008 Sep 17.

Abstract

dATP derivatives bearing Br, Me or Ph groups in position 8 were prepared and tested as substrates for DNA polymerases to show that 8-Br-dATP and 8-Me-dATP were efficiently incorporated, while 8-Ph-dATP was a poor substrate due to its bulky Ph group.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Circular Dichroism
  • DNA / genetics
  • DNA / metabolism*
  • DNA-Directed DNA Polymerase / metabolism*
  • Deoxyadenine Nucleotides / chemical synthesis*
  • Deoxyadenine Nucleotides / chemistry
  • Deoxyadenine Nucleotides / metabolism*
  • Nucleic Acid Denaturation
  • Transition Temperature

Substances

  • Deoxyadenine Nucleotides
  • DNA
  • DNA-Directed DNA Polymerase
  • 2'-deoxyadenosine triphosphate