Multiplex ABO genotyping by minisequencing

Gianmarco Ferri; Susi Pelotti

doi:10.1007/978-1-59745-553-4_5

Multiplex ABO genotyping by minisequencing

Methods Mol Biol. 2009:496:51-8. doi: 10.1007/978-1-59745-553-4_5.

Authors

Gianmarco Ferri¹, Susi Pelotti

Affiliation

¹ Department of Diagnostic and Laboratory Service, Section of Legal Medicine, University of Modena Reggio Emilia, Italy.

PMID: 18839104
DOI: 10.1007/978-1-59745-553-4_5

Abstract

The minisequencing multiplex reaction to genotype ABO blood group system is described and discussed. This method was found to be a reproducible strategy to type 6 common alleles (A1, A2, B, 01, 01V, and 02) of the ABO blood group system, the high specificity and sensitivity make it suitable also in forensic science. It is more rapid than RFLP and SSCP analysis, resulting in unambiguous interpretation of ABO genotypes and newly discovered mutations are readily investigated by the addition of new extension primers in the minisequencing multiplex reaction.

Publication types

Review

MeSH terms

ABO Blood-Group System / genetics*
Alleles
Blood Grouping and Crossmatching / methods*
DNA Primers / genetics
Forensic Genetics / methods
Genotype
Humans
Polymerase Chain Reaction / methods*
Polymorphism, Restriction Fragment Length*
Polymorphism, Single-Stranded Conformational*
Sequence Analysis, DNA / methods*

Substances

ABO Blood-Group System
DNA Primers