Thin frozen film method for visualization of storage proteins in mature rice grains

Biosci Biotechnol Biochem. 2008 Oct;72(10):2779-81. doi: 10.1271/bbb.80337. Epub 2008 Oct 7.

Abstract

There are technical difficulties in obtaining intact sections of cereal grains in which mature cells and their subcellular structures are well preserved. Here we describe a simple method for sectioning hard mature rice grains. It makes possible accurate localization of storage proteins in high-quality histological sections of rice endosperm.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Frozen Sections / methods*
  • Oryza* / growth & development
  • Plant Proteins*

Substances

  • Plant Proteins