Abstract
Excessive nitric oxide (NO) production by activated microglial cells has been implicated in various neurodegenerative diseases. In the present study, we found that a new resveratrol derivative, (E)-5-(3-nitrostyryl)benzene-1,3-diol (RV06), has a more potential inhibitory effect on the production of NO in LPS-activated N9 microglial cells, and the result was confirmed on primary rat microglial cells. Further studies showed that RV06 inhibited LPS-induced iNOS expression in N9 microglial cells, with no activity on direct scavenging nitric oxide radical in a cell-free environment. The results suggest that RV06 might be a potential anti-inflammatory agent or leading compound which can inhibit inflammatory responses of microglia.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Blotting, Western
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Cell Line
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Cell Survival / drug effects
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Free Radical Scavengers / pharmacology*
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Humans
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Indicators and Reagents
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Lipopolysaccharides / pharmacology*
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Microglia / drug effects*
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Microglia / metabolism
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Nitric Oxide / biosynthesis*
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Nitric Oxide Donors / pharmacology
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Nitric Oxide Synthase Type II / antagonists & inhibitors
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Nitrites / metabolism
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Nitroprusside / pharmacology
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Reactive Nitrogen Species / metabolism
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Resveratrol
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Stilbenes / pharmacology*
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Free Radical Scavengers
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Indicators and Reagents
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Lipopolysaccharides
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Nitric Oxide Donors
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Nitrites
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Reactive Nitrogen Species
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Stilbenes
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Nitroprusside
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Nitric Oxide
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Nitric Oxide Synthase Type II
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Resveratrol