On the measurement of the functional properties of the CFTR

Oscar Moran; Olga Zegarra-Moran

doi:10.1016/j.jcf.2008.05.003

On the measurement of the functional properties of the CFTR

J Cyst Fibros. 2008 Nov;7(6):483-94. doi: 10.1016/j.jcf.2008.05.003. Epub 2008 Sep 25.

Authors

Oscar Moran¹, Olga Zegarra-Moran

Affiliation

¹ Istituto di Biofisica, CNR, 16149 Genova, Italy. moran@ge.ibf.cnr.it

PMID: 18818127
DOI: 10.1016/j.jcf.2008.05.003

Abstract

A number of methods are currently employed to assess the functional properties of CFTR channels and their response to pharmacological potentiators, correction of the defective CFTR trafficking, and vectorial introduction of new proteins. Here we review the most common methods used to assess CFTR channel function. The suitability of each technique to various experimental conditions is discussed.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Cystic Fibrosis / etiology
Cystic Fibrosis / metabolism*
Cystic Fibrosis / pathology
Cystic Fibrosis Transmembrane Conductance Regulator / physiology*
Humans
Membrane Potentials / physiology
Protein Transport / physiology
Signal Transduction / physiology

Substances

CFTR protein, human
Cystic Fibrosis Transmembrane Conductance Regulator