Abstract
The Notch signaling pathway (NSP) is an important intercellular communication mechanism that regulates embryo development and adult physiological functions. The Hairless (H) protein is a powerful antagonist of the NSP by its interaction with the Suppressor of Hairless (Su[H]) protein, recruiting the corepressors Gro and CtBP. In the present work, we examined the role of several important amino acids in different H protein domains analyzing four mutant lines of Drosophila melanogaster. The mutant alleles were evaluated by single-strand conformational polymorphism (SSCP) analysis and we located mutated regions at different positions along the sequence of the Hairless gene.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Drosophila Proteins / chemistry*
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Drosophila Proteins / genetics*
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Drosophila Proteins / metabolism
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Drosophila melanogaster / chemistry
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Drosophila melanogaster / genetics
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Drosophila melanogaster / metabolism
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Molecular Sequence Data
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Point Mutation / genetics*
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Point Mutation / physiology
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Polymorphism, Single-Stranded Conformational / genetics
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Receptors, Notch / genetics
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Signal Transduction / genetics
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Transcription Factors / chemistry*
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Transcription Factors / genetics*
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Transcription Factors / metabolism
Substances
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Drosophila Proteins
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N protein, Drosophila
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Receptors, Notch
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Transcription Factors
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H protein, Drosophila