[Cloning and characterization of the chloramphenicol resistant gene of Serratia marcescens strain KMR-3]

Xiuling Ji; Lianbing Lin; Shenrong Jing; Yunlin Wei

[Cloning and characterization of the chloramphenicol resistant gene of Serratia marcescens strain KMR-3]

Sheng Wu Gong Cheng Xue Bao. 2008 Jun;24(6):1016-21.

[Article in Chinese]

Authors

Xiuling Ji¹, Lianbing Lin, Shenrong Jing, Yunlin Wei

Affiliation

¹ Biotechnology Research Center of Kunming University of Science and Technology, Kunming 650224, China.

PMID: 18807985

Abstract

Chloramphenicol-resistant gene was cloned and analyzed by constructing genomic DNA library of Serratia marcescens KMR-3. It showed that cloned chloramphenicol-resistant gene encoded a protein product of 397 amino acids. The protein belonged to PRK10473 protein, and it showed 92% similarity to drug resistance transporter, Bcr/CflA subfamily of Serratia proteamaculans 568. Regulation elements including promoter, terminator, Shine-Dalgarno (SD) sequence and transcription start site also were identified.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
Chloramphenicol Resistance / genetics*
Cloning, Molecular
Molecular Sequence Data
Serratia marcescens / classification
Serratia marcescens / genetics*