The objective of this work is to study the biochemical effect in liver and kidney 2, 14, and 28 days after applying different tissue adhesives. In a prospective longitudinal study, 20 adult rats are assigned to two groups. After making incisions in the buccal mucosa, n-butyl-2-cyanoacrylate is applied to the first group and 2-octyl-cyanoacrylate (OCA) to the second. The biochemical parameters studied are: albumin (ALB), alanine aminotransferase (ALT), amylase (AMY), total bilirubin (TBI), blood urea nitrogen (BUN), creatinine (CRE), glucose, globulin, total proteins (TP), and the ions calcium, phosphorus, sodium, and potassium. The group treated with n-butyl-2-cyanoacrylate shows no statistically significant differences with respect to the control, but OCA produces statistically significant alterations in BUN, ALT, TP, TBI, and AMY. The application of n-butyl-2-cyanoacrylate to wounds in the buccal mucosa produces no significant alterations in the hepatic and renal profile of the animals studied, while OCA produces greater changes in the biochemical profile.