Electron paramagnetic resonance spin trapping of glutathiyl radicals by PBN in the presence of cyclodextrins and by PBN attached to beta-cyclodextrin

Dmitriy N Polovyanenko; Sylvain R A Marque; Sandrine Lambert; Laszlo Jicsinszky; Victor F Plyusnin; Elena G Bagryanskaya

doi:10.1021/jp8050164

Electron paramagnetic resonance spin trapping of glutathiyl radicals by PBN in the presence of cyclodextrins and by PBN attached to beta-cyclodextrin

J Phys Chem B. 2008 Oct 16;112(41):13157-62. doi: 10.1021/jp8050164. Epub 2008 Sep 18.

Authors

Dmitriy N Polovyanenko¹, Sylvain R A Marque, Sandrine Lambert, Laszlo Jicsinszky, Victor F Plyusnin, Elena G Bagryanskaya

Affiliation

¹ International Tomography Center SB RAS, Novosibirsk, Russia.

PMID: 18798667
DOI: 10.1021/jp8050164

Abstract

The reaction of the glutathiyl radical (GS*) with a widely used spin trap N- tert-butyl-alpha-phenylnitrone (PBN) has been studied in the presence of various methylated beta-cyclodextrins and with PBN covalently bound to dimethylated beta-cyclodextrin (PBN-DIMEB) and permethylated beta-cyclodextrin (PBN-TRIMEB). Scavenging rate constants for GS* by PBN were obtained in the presence of randomly methylated cyclodextrin (RAMEB) and PBN-TRIMEB and found to be close to the rate constant previously measured for PBN. RAMEB and 2,6-di- O-Me-beta-cyclodextrin (DIMEB) were found to be the most efficient in the increasing PBN/GS* lifetime, by a factor of 5.5 for RAMEB and 6.8 for DIMEB compared with the lifetime of PBN/GS*. It is concluded that the presence or "attachment of" beta-cyclodextrins does not influence the scavenging rate constant of GS* but it does lead to stabilization of the spin adducts formed.