Abstract
The nucleotide sequence of a 876 bp region in E. coli chromosome that encodes Ecotin was determined. The proposed coding sequence for Ecotin is 486 nucleotides long, which would encode a protein consisting of 162 amino acids with a calculated molecular weight of 18,192 Da. The deduced primary sequence of Ecotin includes a 20-residue signal sequence, cleavage of which would give rise to a mature protein with a molecular weight of 16,099 Da. Ecotin does not contain any consensus reactive site sequences of known serine protease inhibitor families, suggesting that Ecotin is a novel inhibitor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Base Sequence
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Cloning, Molecular*
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Escherichia coli / genetics*
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Escherichia coli Proteins*
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Molecular Sequence Data
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Molecular Weight
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Periplasmic Proteins*
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Protein Sorting Signals / chemistry
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Restriction Mapping
Substances
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Bacterial Proteins
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Eco protein, E coli
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Escherichia coli Proteins
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Periplasmic Proteins
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Protein Sorting Signals
Associated data
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GENBANK/X58797
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GENBANK/X58798
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GENBANK/X58799
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GENBANK/X58800
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GENBANK/X58801
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GENBANK/X58802
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GENBANK/X58803
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GENBANK/X61951
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GENBANK/X61953
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GENBANK/X62841