Differential mass spectrometry of rat plasma reveals proteins that are responsive to 17beta-estradiol and a selective estrogen receptor modulator PPT

J Proteome Res. 2008 Oct;7(10):4373-83. doi: 10.1021/pr800309z. Epub 2008 Sep 12.

Abstract

Estrogens are a class of steroid hormones that interact with two related but distinct nuclear receptors, estrogen receptor (ER) alpha and beta. To identify potential ER biomarkers, we profiled the rat plasma glycoproteome after treatment with vehicle or 17beta-estradiol (E2) or an ERalpha-selective agonist PPT by differential mass spectrometry. Our comparative proteomic experiment identifies novel E2- and PPT-responsive proteins, such as serine protease inhibitor family members.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blood Proteins / chemistry
  • Blood Proteins / genetics
  • Blood Proteins / metabolism*
  • Estradiol / metabolism*
  • Female
  • Glycoproteins / chemistry
  • Glycoproteins / genetics
  • Glycoproteins / metabolism
  • Male
  • Mass Spectrometry / methods*
  • Molecular Sequence Data
  • Organ Size
  • Peptides / chemistry
  • Peptides / genetics
  • Peptides / metabolism
  • Phenols / metabolism*
  • Plasma / chemistry*
  • Pyrazoles / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Estrogen / agonists
  • Receptors, Estrogen / metabolism
  • Selective Estrogen Receptor Modulators / metabolism*
  • Uterus / anatomy & histology

Substances

  • Blood Proteins
  • Glycoproteins
  • Peptides
  • Phenols
  • Pyrazoles
  • Receptors, Estrogen
  • Selective Estrogen Receptor Modulators
  • 4,4',4''-(4-propyl-((1)H)-pyrazole-1,3,5-triyl) tris-phenol
  • Estradiol