Dynamic imaging of calcium and STIM1 in the same cell using wide-field and TIRF microscopy

Biotechniques. 2008 Sep;45(3):347-8. doi: 10.2144/000112957.
No abstract available

MeSH terms

  • Calcium / metabolism*
  • Calcium Signaling / physiology*
  • Cell Line
  • Cell Membrane / metabolism
  • Fluorescent Dyes / metabolism
  • Fura-2 / analogs & derivatives
  • Fura-2 / metabolism
  • Humans
  • Kidney / cytology
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Microscopy, Fluorescence*
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / metabolism*
  • Stromal Interaction Molecule 1
  • Thapsigargin / pharmacology
  • Transfection

Substances

  • Fluorescent Dyes
  • Membrane Proteins
  • Neoplasm Proteins
  • STIM1 protein, human
  • Stromal Interaction Molecule 1
  • fura-2-am
  • Thapsigargin
  • Calcium
  • Fura-2