Conversion of 5-hydroxytryptophan into serotonin by tryptophan decarboxylase in plants, Escherichia coli, and yeast

Munyoung Park; Kiyoon Kang; Sangkyu Park; Kyoungwhan Back

doi:10.1271/bbb.80220

Conversion of 5-hydroxytryptophan into serotonin by tryptophan decarboxylase in plants, Escherichia coli, and yeast

Biosci Biotechnol Biochem. 2008 Sep;72(9):2456-8. doi: 10.1271/bbb.80220. Epub 2008 Sep 7.

Authors

Munyoung Park¹, Kiyoon Kang, Sangkyu Park, Kyoungwhan Back

Affiliation

¹ Department of Molecular Biotechnology, Agricultural Plant Stress Research Center, Biotechnology Research Institute, Chonnam National University, Gwangju, Japan.

PMID: 18776677
DOI: 10.1271/bbb.80220

Abstract

The L-tryptophan decarboxylase (TDC) gene of rice was heterologously expressed in various organisms. Transgenic rice overexpressing TDC showed accumulation of serotonin upon 5-hydroxytryptophan treatment, which was consistent with the in vitro 5-hydroxytryptophan decarboxylase enzyme activity of purified recombinant rice TDC in a pyridoxal phosphate-dependent manner. Recombinant yeast harboring TDC produced serotonin at the expense of the endogenous 5-hydroxytryptophan levels.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

5-Hydroxytryptophan / metabolism
Aromatic-L-Amino-Acid Decarboxylases / genetics*
Aromatic-L-Amino-Acid Decarboxylases / metabolism
Escherichia coli / genetics*
Escherichia coli / metabolism
Oryza / genetics
Oryza / metabolism
Plants / genetics*
Plants / metabolism
Plants, Genetically Modified / genetics
Plants, Genetically Modified / metabolism
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / metabolism
Serotonin / genetics*
Yeasts / genetics
Yeasts / metabolism

Substances

Serotonin
5-Hydroxytryptophan
Aromatic-L-Amino-Acid Decarboxylases