Fluorescent ligand as a molecular probe for the RNA structure

Nucleic Acids Symp Ser (Oxf). 2008:(52):211-2. doi: 10.1093/nass/nrn107.

Abstract

In this report, we show the recognition of RNA secondary structure by exploiting a fluorescent 2,7-disubstituted 9H-xanthen-9-one derivative (2). The xanthone derivertive (2) especially bound to RNA and became non-fluorescent. Upon mixing with RRE model RNA, U-bulge RNA, or hairpin RNA, the fluorescence intensity of 2 was decreased in different intensity. The distinct fluorescent increase of 2 to each RNA seems to a good probe for The RNA structures.

MeSH terms

  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Ligands
  • Nucleic Acid Conformation
  • RNA / chemistry*
  • Spectrometry, Fluorescence
  • Xanthones / chemical synthesis
  • Xanthones / chemistry*

Substances

  • 2,7-bisaminoethoxyxanthen-9-one
  • Fluorescent Dyes
  • Ligands
  • Xanthones
  • RNA