Flow cytometric analysis of cytokine responses in stimulated whole blood: simultaneous quantitation of TNF-alpha-secreting cells and soluble cytokines

Curr Protoc Cytom. 2003 Aug:Chapter 9:9.21.1-9.21.21. doi: 10.1002/0471142956.cy0921s25.

Abstract

This unit describes technical protocols aimed at the ex vivo or in vitro evaluation of the functional status of the immune system through the simultaneous identification and enumeration of cytokine-secreting cells and quantitation of the soluble cytokines produced by these cells.

MeSH terms

  • Biomedical Research / methods
  • Blood Cells
  • Cytokines / analysis*
  • Flow Cytometry / methods*
  • Humans
  • Immune System
  • Leukocytes / metabolism*
  • Models, Biological
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Cytokines
  • Tumor Necrosis Factor-alpha