During an influenza pandemic, public health staff may not report to work due to illness, transport disruptions or care responsibilities, including care of children if school closures occur. A survey was conducted in a population health unit to estimate the impact of closure of schools and day care facilities on staff ability to work at their usual workplace or at home, and determine their access to the Internet for communication. Staff were also asked about concerns associated with working from home. Eighty-seven staff completed a paper based survey. Thirty-eight per cent (33/87) of staff may be absent from work due to the impact of childcare and school closure, however 24 (73%) of these staff would be able to work from home with most having access to dial-up (87%) and broadband Internet access (71%). Staff reported concerns about potential exposure to pandemic influenza, the need for personal protection and clearly defined roles and training, availability of adequate equipment and technology to work from home, and sick leave provisions during a pandemic. While school and childcare closures will have a significant impact on public health agency staff, they have the capacity and willingness to work from home. Their practical concerns should be addressed to optimise their participation.