Abstract
A novel gene, bla(KHM-1), encoding a metallo-beta-lactamase, KHM-1, was cloned from a clinical isolate of Citrobacter freundii resistant to most beta-lactam antibiotics. Escherichia coli expressing bla(KHM-1) was resistant to all broad-spectrum beta-lactams except for monobactams and showed reduced susceptibility to carbapenems. Recombinant KHM-1 exhibited EDTA-inhibitable hydrolytic activity against most beta-lactams, with an overall preference for cephalosporins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Citrobacter freundii / drug effects
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Citrobacter freundii / enzymology*
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Citrobacter freundii / genetics*
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Citrobacter freundii / isolation & purification
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Conjugation, Genetic
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DNA Primers / genetics
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DNA, Bacterial / genetics
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Enterobacteriaceae Infections / drug therapy
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Enterobacteriaceae Infections / microbiology
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Escherichia coli K12 / drug effects
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Escherichia coli K12 / genetics
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Genes, Bacterial
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Humans
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Phylogeny
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Plasmids / genetics
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Sequence Homology, Amino Acid
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beta-Lactam Resistance / genetics
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beta-Lactamases / genetics*
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beta-Lactamases / metabolism
Substances
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DNA Primers
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DNA, Bacterial
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Recombinant Proteins
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beta-Lactamases