Comparison of "Polarization inversion with spin exchange at magic angle" and "geometric analysis of labeled alanines" methods for transmembrane helix alignment

Vitaly V Vostrikov; Christopher V Grant; Anna E Daily; Stanley J Opella; Roger E Koeppe 2nd

doi:10.1021/ja803734k

Comparison of "Polarization inversion with spin exchange at magic angle" and "geometric analysis of labeled alanines" methods for transmembrane helix alignment

J Am Chem Soc. 2008 Sep 24;130(38):12584-5. doi: 10.1021/ja803734k. Epub 2008 Sep 3.

Authors

Vitaly V Vostrikov¹, Christopher V Grant, Anna E Daily, Stanley J Opella, Roger E Koeppe 2nd

Affiliation

¹ Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, USA.

Abstract

Using the model alpha-helical peptide acetyl-GGALW5LALALALALALALW19LAGA-ethanolamide ("GWALP23"), we have compared the polarization inversion with spin exchange at magic angle method and geometric analysis of labeled alanines method for estimating the transmembrane helix orientation. For GWALP23 in bilayers of a short lipid, dilauroylphosphatidylcholine, we find general agreement between the two methods, with a static helix tilt of about 11degrees-13degrees with respect to the bilayer normal.

Publication types

Comparative Study
Research Support, N.I.H., Extramural

MeSH terms

Alanine / analogs & derivatives*
Alanine / chemistry
Amides / chemistry
Ethanol / analogs & derivatives
Lipid Bilayers / chemistry
Nuclear Magnetic Resonance, Biomolecular / methods*
Peptides / chemistry*
Phosphatidylcholines / chemistry
Protein Structure, Secondary

Substances

Amides
Lipid Bilayers
Peptides
Phosphatidylcholines
1,2-dilauroylphosphatidylcholine
Ethanol
Alanine

Abstract

Publication types

MeSH terms

Substances

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