The effect of extra-cellular polymeric substances (EPS) on filtration of polyvinyl alcohol modified polypropylene non-woven in submerged membrane bioreactor (SMBR) was investigated by statistical method. The results show that soluble extra-cellular polymeric substances (EPSs) of activated sludge on the non-woven modules surface, components (protein/carbohydrate, P/C) of EPSs and relative hydrophobicity (RH) have a significant influence on filtration performance of module B, the Pearson's correlation coefficient (r(p)) related to membrane fouling resistance are 0.868, 0.840, 0.890, respectively. Modified module can effectively restrict the adsorption of EPSs, can reduce the ratio of P/C in EPSs and can decrease the accumulation of activated sludge. After hydrophilic modification of non-woven, the filtration performance is improved obviously and the un-fouling performance is increased.