Chemical constituents from the leaves of Manglietia phuthoensis and their effects on osteoblastic MC3T3-E1 cells

Chem Pharm Bull (Tokyo). 2008 Sep;56(9):1270-5. doi: 10.1248/cpb.56.1270.

Abstract

Two new phenyl glycosides, mangliesides A and B (1, 2), a new ionol glycoside, manglieside C (3), two new lignan glycosides, mangliesides D and E (4, 5), were isolated from the leaves of Manglietia phuthoensis, along with two known lignans, 3-methoxymagnolol (6) and obovatol (7). Their structures were established by means of 1D and 2D NMR, electrospray ionization (ESI)-MS and HR-ESI-MS experiments. Among them, compounds 2 and 5 significantly (p<0.05) increased the growth and differentiation of osteoblastic MC3T3-E1 cells in vitro.

MeSH terms

  • 3T3 Cells
  • Alkaline Phosphatase / metabolism
  • Animals
  • Bone Density Conservation Agents / isolation & purification*
  • Bone Density Conservation Agents / pharmacology*
  • Calcium / metabolism
  • Cell Differentiation / drug effects
  • Cell Survival / drug effects
  • Collagen / analysis
  • Collagen / biosynthesis
  • Estradiol / chemistry
  • Estradiol / pharmacology
  • Hydrolysis
  • Magnetic Resonance Spectroscopy
  • Magnolia / chemistry*
  • Mice
  • Models, Molecular
  • Molecular Conformation
  • Osteoblasts / drug effects*
  • Plant Leaves / chemistry
  • Spectrometry, Mass, Electrospray Ionization
  • Spectrophotometry, Infrared

Substances

  • Bone Density Conservation Agents
  • Estradiol
  • Collagen
  • Alkaline Phosphatase
  • Calcium