Artificial transcription factors targeting any desired genes are very attractive but require specific DNA binding domains in order to address a single site for each gene promoter. By connecting various zinc fingers recognizing the corresponding 3-4 bp DNA, DNA binding domains for the desired and long sequences can be created. Though such a long sequence recognition is a marvelous property, we have found that as the number of finger motifs increases, the equilibrium time with the target sequence is significantly longer as detected by in vitro EMSA experiments. In this study, we created 3- and 9-finger-type artificial transcription factors and compared the kinetics of the transcriptional activation in vivo as to whether or not a significant delay in the activation is observed for the 9-finger type. By using a ligand-inducing system, we demonstrated for the first time that finger multimerization does not affect the kinetics of the transcriptional activity; the 9-finger type artificial transcription factor activated the reporter gene as quickly as the 3-figner type. Our results suggest that the drawback of finger multimerization, i.e., the equilibrium time is prolonged depending on the number of finger motifs, can be surmounted in terms of its use for transcription factors in vivo. There is much interest in creating therapeutic molecules, and these findings suggest the significant potential of multi-zinc finger proteins as a tool for an artificial gene regulator.